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KMID : 1094719990040030165
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.165 ~ p.169
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Sialic acids inTrichoplusia ni andDanaus plexippus egg glycoproteins
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Park Yong-Il
Yoon Byung-Dae
Lee Yuan Chuan
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Abstract
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Baculovirus expression vector insect cell system (BEVIS) is useful for high level production of human therapeutic proteins. However, it has been reported that recombinant glycoproteins produced from this system are, in many cases, biologically inactive or less active than authentic counterparts, due to incomplete glycosylation potential of insect cells used so far, producing recombinant proteins with only high-or paucimannosidic oligosaccharides without sialylation. The presence of sialic acids in insects is still controversial. Egg proteins ofTrichoplusia ni andDanaus plexippus were isolated, and the presence of sialic acids was examined using reverse-phase fluorescent HPLC after derivatization of samples with 1,2-diamino-4,5-methylenedioxybenzene (DMB). The proteins of both eggs were shown to contain 5-N-acetylneuraminic acid. The results suggest that both insects may be able to produce proteins with sialylated complex-type oligosaccharide chains.
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KEYWORD
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sialic acids, glycoproteins, carbohydrates, insects, HPLC
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